PPT Slide

16S rDNA based molecular techniques

Denaturing Gradient Gel Electrophoresis of PCR products

• PCR of V3 region of 16S rDNA (F357, R518)

• Separation of PCR products in denaturing gradient gel electrophoresis (DGGE) due to differences in nucleotide composition which cause different melting behaviour

Clone library

• PCR of near complete 16S rDNA + cloning

• DGGE comparison of clones to original sample after V3 PCR

• Sequencing of randomly chosen clones

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